Week 9

This week I learned how to extract DNA from my bacteria using an easy protocol that one of the scholars (Paul Cattelino) has designed. In this method, I used a big amount of my sample (500 µL) to extract DNA from, so it didn’t need to be amplified in order to be seen. I learned that for extracting DNA, we’d better to use a sample that has been cultured overnight and is fresh. Then, I ran PCR with getting help from Paul. I learned to different techniques running PCRs. The difference of two techniques was in the way of loading the DNA sample into the gels. The first technique used was the way that microbiologists use, and it’s purring the buffer first and then loading the sample. The second technique was loading the sample and then purring the buffer which is not as perfect as the first method, but it’s an easier way to load the sample. Finally, I ran the PCR with both methods, but something was done wrong because I didn’t have any DNA extracted from my samples. I will repeat the experiment next week all over again to see if I can get some results.

https://www.youtube.com/watch?v=DkT6XHWne6E