Unknown Lab

This week was basically my first lab experiment. I learned about five I’s: inoculation, incubation, isolation, inspection and identification. I inoculated a sample from TSB (soy broth) to TSA slant (soy agar) using loop and dragging it in “S” pattern back and forth across the surface of the agar. The loop should be sterilized by heating it in Bacti-Cinerator for 5 seconds before each use. I also learned how to streak a plate in two different ways. First, I used the lawn technique. It’s basically spreading your bacteria on a petri-dish in an “S” pattern. I spread my unknown on the first region.Then turned my plate 60 ͦ and kept spreading it and did another 60 ͦ turn to make sure it’s spread really well. Next technique was isolation technique in which I divided a plate to three separate regions. Then, I spread the sample or my unknown on the first region. To go to second region, I got two lines from first region and spread those on my second region. I repeated it for third region, but getting my two lines from second region this time. I incubated them all and waiting for them to grow, so I can inspect and eventually identify my unknown. Bellow is a picture of my plate, slant tube and unknown.