Fifth week

 

 

last week I figured out that my bacteria was Gram Positive Cocci. This week, I did more tests to figure out my unknown. I added hydrogen Peroxide on my bacteria sample for Catalase Test and saw it's bobbling, so I moved to the next step. My bacteria had changed the color of the indicator dye in the glucose and lactose indicating the change of the PH in the medium, and bobbles were trapped in Durham tube. So the result was positive, and I had to look at the result on my MSA Test. Sure enough that it had turned from red to yellow. Now with having a positive result for my last test I can conclude that my unknown is staphylococcus aureus. I also incubated an MSA and a TSA plate with bacteria from my skin. next week, I will extract DNA from the bacteria that I had initially as my unknown and the one that grew from my skin to see if those are the same type of bacteria. It will be exciting if I find the similar result.

Glucose, lactose, and MSA plate after incubating my initial unknown

 MSA and TSA plates before inoculation

 

Grown bacteria that I got from my skin 

 

Inoculated broth with bacteria from MSA plate with grown bacteria from my skin