Week 10

 

This week I learned how to extract DNA using heat. If we have a Gram positive bacteria which has a bilayer cell wall, we can use boiling water to extract the DNA without damaging it. Extracting DNA,  using this technique can be easily done. We need enough amount of a fresh sample. Then we can pipet some of our sample into a tube, and spin it in a centrifuge. Now, pallet can be re-suspended in a new tube with some water in it. Next step is to sit the pallet in a boiling water which will break the cell wall. We, then, centrifuge the tube one more time to pallet the constituent. We keep the supernatant of the last step which is the extracted DNA.

 

                                     DNA extracted at the bottom of the tube (google image)